Characterisation of the high-molecular weight fructan isolated from garlic (Allium sativum L.)

Carbohydr Res. 2000 Sep 8;328(2):177-83. doi: 10.1016/s0008-6215(00)00097-5.

Abstract

A high molecular weight fructan was isolated from garlic and the structure determined by enzymatic, chemical and spectroscopic (NMR) methods. It was found that the garlic fructan belongs to the neokestose family. It has a (2 --> 1)-linked beta-D-Fruf backbone with (2 --> 6)-linked beta-D-Fruf side chains. A structural model was postulated for a degree of polymerisation of about 58. This model was substantiated using an endo-inulinase purified from Aspergillus ficuum and by 1H and 13C NMR spectroscopy.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus / enzymology
  • Carbohydrate Sequence
  • Fructans / chemistry*
  • Fructans / isolation & purification
  • Fructans / metabolism
  • Garlic / chemistry*
  • Glycoside Hydrolases / metabolism
  • Molecular Sequence Data
  • Molecular Weight
  • Nuclear Magnetic Resonance, Biomolecular
  • Oligosaccharides / chemistry
  • Plants, Medicinal*

Substances

  • Fructans
  • Oligosaccharides
  • Glycoside Hydrolases
  • inulinase
  • sinistrin