Genomic organization of a new candidate tumor suppressor gene, LRP1B

Genomics. 2000 Oct 15;69(2):271-4. doi: 10.1006/geno.2000.6331.


LRP1B is a novel candidate tumor suppressor gene that is inactivated by genetic and transcript alterations in nearly 50% of non-small-cell lung cancer cell lines. The gene-encoded protein is highly homologous to the gigantic lipoprotein receptor-related protein 1 (LRP1) that belongs to the family of low-density lipoprotein receptors. Using a combination of PCR-based genome walking and long-distance interexon PCR, we have determined the genomic organization of LRP1B and built a contiguous array of BAC clones spanning this gene. A total of 91 exons, varying in size from 77 bases (exon 87) to 1899 bases (exon 91), were identified in the more than 500-kb-long gene sequence. Comparative analysis of the genomic structures of LRP1B and the homologous LRP1 gene revealed a striking similarity in the location and sizes of their exons.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosomes, Artificial, Bacterial
  • Exons
  • Genes, Tumor Suppressor*
  • Humans
  • Introns
  • Low Density Lipoprotein Receptor-Related Protein-1
  • Molecular Sequence Data
  • Receptors, Immunologic / genetics*
  • Sequence Homology, Nucleic Acid


  • Low Density Lipoprotein Receptor-Related Protein-1
  • Receptors, Immunologic

Associated data

  • GENBANK/AF122013
  • GENBANK/AF283327
  • GENBANK/AF283328
  • GENBANK/AF283329
  • GENBANK/AF283330
  • GENBANK/AF283331
  • GENBANK/AF283332
  • GENBANK/AF283333
  • GENBANK/AF283334
  • GENBANK/AF283335
  • GENBANK/AF283336
  • GENBANK/AF283337
  • GENBANK/AF283338
  • GENBANK/AF283339
  • GENBANK/AF283340
  • GENBANK/AF283341
  • GENBANK/AF283342
  • GENBANK/AF283343
  • GENBANK/AF283344
  • GENBANK/AF283345
  • GENBANK/AF283346
  • GENBANK/AF283347
  • GENBANK/AF283348
  • GENBANK/AF283349
  • GENBANK/AF283350
  • GENBANK/AF283351
  • GENBANK/AF283352
  • GENBANK/AF283353
  • GENBANK/AF283354
  • GENBANK/AF283355