Pulsed-field gel electrophoresis analysis of virus assemblages present in a hypersaline environment

Int Microbiol. 2000 Sep;3(3):159-64.

Abstract

A method for analyzing virus assemblages in aquatic environments was developed and used for studying the highest-salinity ponds (from 13.4 to 35% salinity) from a multi-pond solar saltern in Alicante, Spain. The protocol consisted of a series of concentration and purification steps including tangential flow filtration and ultracentrifugation, followed by the preparation of total viral nucleic acids that were subsequently separated by pulsed-field gel electrophoresis. For every sample analyzed, a characteristic DNA pattern was obtained, whose complexity was related to viral diversity. The comparison of our results with a similar analysis carried out with marine virus assemblages shows that, as expected, the viral diversity corresponding to the analyzed hypersaline environment is considerably lower than that of a marine environment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophages / drug effects
  • Bacteriophages / genetics
  • Bacteriophages / isolation & purification
  • DNA, Viral / analysis*
  • DNA, Viral / isolation & purification
  • Electrophoresis, Gel, Pulsed-Field*
  • Saline Solution, Hypertonic / pharmacology*
  • Spain
  • Virus Physiological Phenomena*
  • Viruses / drug effects*
  • Viruses / genetics
  • Viruses / isolation & purification
  • Water / chemistry*

Substances

  • DNA, Viral
  • Saline Solution, Hypertonic
  • Water