Identification of a novel fibroblast growth factor, FGF-23, preferentially expressed in the ventrolateral thalamic nucleus of the brain

Biochem Biophys Res Commun. 2000 Oct 22;277(2):494-8. doi: 10.1006/bbrc.2000.3696.

Abstract

We isolated mouse cDNA encoding a novel FGF (251 amino acids). As this is the 23rd documented FGF, we termed it FGF-23. FGF-23 has a hydrophobic amino terminus ( approximately 24 amino acids), which is a typical signal sequence. As expected, recombinant mouse FGF-23 was efficiently secreted by High Five insect cell-infected recombinant baculovirus containing the cDNA, indicating that FGF-23 is a secreted protein. We also isolated human cDNA encoding FGF-23 (251 amino acids), which is highly identical ( approximately 72% amino acid identity) to mouse FGF-23. Of human FGF family members, FGF-23 is most similar to FGF-21 and FGF-19 ( approximately 24% and approximately 22% amino acid identities, respectively). Human FGF-23 gene was localized on the chromosome 12p13 and found to be tandem linked (within 5.5 kb) to human FGF-6 gene. The expression of FGF-23 mRNA in mouse adult tissues was examined by real-time quantitative polymerase chain reaction. FGF-23 mRNA was mainly expressed in the brain and thymus at low levels. The localization of FGF-23 mRNA in the brain was examined by in situ hybridization. FGF-23 mRNA in the brain was found to be preferentially expressed in the ventrolateral thalamic nucleus. Therefore, FGF-23 is expected a unique FGF that plays roles in the function of the ventrolateral thalamic nucleus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Baculoviridae / metabolism
  • Blotting, Western
  • Brain / metabolism*
  • Cell Line
  • Chromosome Mapping
  • Chromosomes, Human, Pair 12
  • DNA, Complementary / metabolism
  • Electrophoresis, Polyacrylamide Gel
  • Fibroblast Growth Factor-23
  • Fibroblast Growth Factors / biosynthesis*
  • Fibroblast Growth Factors / chemistry
  • Fibroblast Growth Factors / genetics
  • Humans
  • In Situ Hybridization
  • Mice
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Complementary / metabolism
  • RNA, Messenger / metabolism
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Ventral Thalamic Nuclei / metabolism*

Substances

  • DNA, Complementary
  • FGF19 protein, human
  • FGF23 protein, human
  • Fgf23 protein, mouse
  • RNA, Complementary
  • RNA, Messenger
  • Recombinant Proteins
  • fibroblast growth factor 21
  • Fibroblast Growth Factors
  • Fibroblast Growth Factor-23

Associated data

  • GENBANK/AB037889
  • GENBANK/AB037973