Abscisic acid plasmalemma perception triggers a calcium influx essential for RAB18 gene expression in Arabidopsis thaliana suspension cells

FEBS Lett. 2000 Oct 13;483(1):67-70. doi: 10.1016/s0014-5793(00)02088-3.

Abstract

Pretreatment of Arabidopsis thaliana suspension cells with impermeant calcium chelator EGTA inhibited the ABA-induced RAB18 gene expression. However, extracellular calcium alone, up to 10 mM, did not trigger RAB18 expression. Spectrofluorimetric extracellular Ca(2+) measurement with Fluo-3 showed a fast, within 1 min, Ca(2+) influx associated with outer plasmalemma ABA perception. In the presence of the Ca(2+) blockers Cd(2+) and Ni(2+), RAB18 expression was suppressed. Pimozide and fluspirilene inhibited Ca(2+) influx and ABA-induced RAB18 expression. Thus we demonstrated the involvement of specific Ca(2+) influx in the ABA signaling sequence leading to RAB18 expression.

MeSH terms

  • Abscisic Acid / pharmacology*
  • Arabidopsis / cytology
  • Arabidopsis / drug effects*
  • Arabidopsis / genetics
  • Arabidopsis Proteins*
  • Biological Transport / drug effects
  • Blotting, Northern
  • Calcium / metabolism*
  • Cell Membrane / drug effects*
  • Cell Membrane / metabolism
  • Dose-Response Relationship, Drug
  • Extracellular Space / drug effects
  • Extracellular Space / metabolism
  • Gene Expression Regulation, Plant / drug effects
  • Plant Proteins / genetics*
  • RNA, Plant / drug effects
  • RNA, Plant / genetics
  • RNA, Plant / metabolism
  • Time Factors
  • rab GTP-Binding Proteins*

Substances

  • Arabidopsis Proteins
  • Plant Proteins
  • RNA, Plant
  • rab18 protein, Arabidopsis
  • Abscisic Acid
  • rab GTP-Binding Proteins
  • Calcium