LEAP-1, a novel highly disulfide-bonded human peptide, exhibits antimicrobial activity

FEBS Lett. 2000 Sep 1;480(2-3):147-50. doi: 10.1016/s0014-5793(00)01920-7.


We report the isolation and characterization of a novel human peptide with antimicrobial activity, termed LEAP-1 (liver-expressed antimicrobial peptide). Using a mass spectrometric assay detecting cysteine-rich peptides, a 25-residue peptide containing four disulfide bonds was identified in human blood ultrafiltrate. LEAP-1 expression was predominantly detected in the liver, and, to a much lower extent, in the heart. In radial diffusion assays, Gram-positive Bacillus megaterium, Bacillus subtilis, Micrococcus luteus, Staphylococcus carnosus, and Gram-negative Neisseria cinerea as well as the yeast Saccharomyces cerevisiae dose-dependently exhibited sensitivity upon treatment with synthetic LEAP-1. The discovery of LEAP-1 extends the known families of mammalian peptides with antimicrobial activity by its novel disulfide motif and distinct expression pattern.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Anti-Bacterial Agents
  • Anti-Infective Agents / classification
  • Anti-Infective Agents / pharmacology*
  • Antimicrobial Cationic Peptides
  • Bacillus megaterium / drug effects
  • Bacillus subtilis / drug effects
  • Base Sequence
  • DNA, Complementary
  • Disulfides
  • Hepcidins
  • Humans
  • Microbial Sensitivity Tests
  • Micrococcus luteus / drug effects
  • Molecular Sequence Data
  • Neisseria / drug effects
  • Peptides / classification
  • Peptides / genetics
  • Peptides / pharmacology*
  • Proteins / classification
  • Proteins / genetics
  • Proteins / pharmacology*
  • Saccharomyces cerevisiae / drug effects
  • Sequence Analysis, DNA
  • Staphylococcus / drug effects


  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Antimicrobial Cationic Peptides
  • DNA, Complementary
  • Disulfides
  • HAMP protein, human
  • Hepcidins
  • Peptides
  • Proteins

Associated data

  • GENBANK/AD000684
  • GENBANK/AJ277280
  • GENBANK/P81172