Linseed oil suppresses the anti-beta-2-glycoprotein-I in experimental antiphospholipid syndrome

J Autoimmun. 2000 Nov;15(3):381-5. doi: 10.1006/jaut.2000.0439.


The immunomodulatory potential of a diet enriched with n-3 polyunsaturated fatty acids was analysed in naïve mice with experimental antiphospholipid syndrome (APS) induced by active immunization with H-3, a human anti-beta-2-glycoprotein-I (anti-betaGPI) monoclonal antibody (mAb). Fetal loss and other clinical manifestations of APS were prevented in the group of APS mice upon exposure to the enriched n-3 diet compared to the control group. The titers of anti-betaGPI were significantly lower (in O.D. at 405 nm, 1.387+/-0.232 in comparison to non-treated mice 0.637+/-0. 111, P< 0.05). The reduced titer of anti-betaGPI antibodies in the sera of the treated mice was associated with a reduced number of anti-betaGPI forming cells in cultured splenocytes (84+/-14, antibody-forming cells (AFC)/10(5)cells in comparison to 37+/-4 AFC/10(5)cells (P< 0.02).In addition to the suppression of the humoral response in mice with experimental APS fed with linseed oil, we also observed an inhibitory effect on the cellular response. The T-cell response to anti-betaGPI was lower in comparison to mice immunized with H-3 anti-betaGPI mAB, which were kept on a normal diet. These results indicate that polyunsaturated fatty acids may improve clinical and laboratory parameters of APS. The beneficial effects of diets enriched with n-3 should be further examined as a potential mode of therapy for patients with APS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / administration & dosage
  • Antibodies, Monoclonal / immunology
  • Antiphospholipid Syndrome / immunology*
  • Apolipoproteins / immunology*
  • Cells, Cultured
  • Dietary Fats
  • Dietary Supplements
  • Disease Models, Animal
  • Fatty Acids / metabolism
  • Female
  • Glycoproteins / immunology*
  • Humans
  • Linseed Oil* / pharmacology
  • Lymph Nodes
  • Membrane Glycoproteins / immunology*
  • Mice
  • Mice, Inbred BALB C
  • beta 2-Glycoprotein I


  • Antibodies, Monoclonal
  • Apolipoproteins
  • Dietary Fats
  • Fatty Acids
  • Glycoproteins
  • Membrane Glycoproteins
  • beta 2-Glycoprotein I
  • Linseed Oil