Specific interactions among transmembrane 4 superfamily (TM4SF) proteins and phosphoinositide 4-kinase

Biochem J. 2000 Nov 1;351 Pt 3(Pt 3):629-37.


In earlier work we established that phosphoinositide 4-kinase (PI 4-kinase) may associate with transmembrane 4 superfamily (TM4SF, tetraspanin) proteins, but critical specificity issues were not addressed. Here we demonstrate that at least five different TM4SF proteins (CD9, CD63, CD81, CD151 and A15/TALLA1) can associate with a similar or identical 55 kDa type II PI 4-kinase. These associations were specific, since we found no evidence for other phosphoinositide kinases (e.g. phosphoinositide 3-kinase and phosphoinositide-4-phosphate 5-kinase) associating with TM4SF proteins, and many other TM4SF proteins (including CD82 and CD53) did not associate with PI 4-kinase. CD63-PI 4-kinase complexes were almost entirely intracellular, and thus are distinct from other TM4SF-PI 4-kinase complexes (e.g. involving CD9), which are largely located in the plasma membrane. These results suggest that a specific subset of TM4SF proteins may recruit PI 4-kinase to specific membrane locations, and thereby influence phosphoinositide-dependent signalling.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 1-Phosphatidylinositol 4-Kinase / chemistry
  • 1-Phosphatidylinositol 4-Kinase / metabolism*
  • Amino Acid Sequence
  • Blotting, Western
  • Cell Line
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Precipitin Tests
  • Protein Binding
  • Substrate Specificity


  • Membrane Proteins
  • 1-Phosphatidylinositol 4-Kinase