Pantetheinase activity of membrane-bound Vanin-1: lack of free cysteamine in tissues of Vanin-1 deficient mice

FEBS Lett. 2000 Oct 20;483(2-3):149-54. doi: 10.1016/s0014-5793(00)02110-4.


Pantetheinase (EC 3.5.1.-) is an ubiquitous enzyme which in vitro has been shown to recycle pantothenic acid (vitamin B5) and to produce cysteamine, a potent anti-oxidant. We show that the Vanin-1 gene encodes pantetheinase widely expressed in mouse tissues: (1) a pantetheinase activity is specifically expressed by Vanin-1 transfectants and is immunodepleted by specific antibodies; (2) Vanin-1 is a GPI-anchored pantetheinase, and consequently an ectoenzyme; (3) Vanin-1 null mice are deficient in membrane-bound pantetheinase activity in kidney and liver; (4) in these organs, a major metabolic consequence is the absence of detectable free cysteamine; this demonstrates that membrane-bound pantetheinase is the main source of cysteamine in tissues under physiological conditions. Since the Vanin-1 molecule was previously shown to be involved in the control of thymus reconstitution following sublethal irradiation in vivo, this raises the possibility that Vanin/pantetheinase might be involved in the regulation of some immune functions maybe in the context of the response to oxidative stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amidohydrolases / genetics
  • Amidohydrolases / metabolism*
  • Animals
  • Blotting, Northern
  • Cell Adhesion Molecules / deficiency
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / metabolism*
  • Cell Line
  • Cysteamine / metabolism
  • GPI-Linked Proteins
  • Gene Expression
  • Gene Expression Regulation, Enzymologic
  • In Situ Hybridization
  • Kidney / chemistry
  • Kidney / enzymology
  • Liver / chemistry
  • Liver / enzymology
  • Membrane Proteins / metabolism*
  • Mice
  • Mice, Inbred Strains
  • Mice, Knockout
  • Phylogeny
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Tissue Distribution


  • Cell Adhesion Molecules
  • GPI-Linked Proteins
  • Membrane Proteins
  • RNA, Messenger
  • Cysteamine
  • Amidohydrolases
  • pantetheinase