Multiplex PCR amplification of TH01, D9S304, and D3S1744 loci

K Fujii; H Senju; K Yoshida; K Sekiguchi; K Imaizumi; K Kasai; H Sato

doi:10.1007/s100380070021

Multiplex PCR amplification of TH01, D9S304, and D3S1744 loci

J Hum Genet. 2000;45(5):303-4. doi: 10.1007/s100380070021.

Authors

K Fujii¹, H Senju, K Yoshida, K Sekiguchi, K Imaizumi, K Kasai, H Sato

Affiliation

¹ Third Medico-Legal Section, National Research Institute of Police Science, Chiba, Japan. fujii@nrips.go.jp

PMID: 11043514
DOI: 10.1007/s100380070021

Abstract

A multiplex typing method of the tetrameric short tandem repeat (STR) loci TH11, D9S304, and D3S1744 was developed. The allelic ladder included alleles 6-11 (80-100bp) and 9.3 (95bp) for TH01, alleles 6-15 (125-161 bp) for D9S304, and alleles 13-22 (174-210bp) for D3S1744. The observed heterozygosity of D9S304 was 0.851. The combined discrimination power of the three loci was 0.991.

MeSH terms

Base Sequence
DNA Primers
Heterozygote
Polymerase Chain Reaction / methods*
Tandem Repeat Sequences / genetics*

Substances

DNA Primers