Recurrent mossy fibers preferentially innervate parvalbumin-immunoreactive interneurons in the granule cell layer of the rat dentate gyrus

Neuroreport. 2000 Sep 28;11(14):3219-25. doi: 10.1097/00001756-200009280-00034.


Detection of vesicular zinc and immunohistochemistry against markers for different interneuron subsets were combined to study the postsynaptic target selection of zinc-containing recurrent mossy fiber collaterals in the dentate gyrus. Mossy fiber collaterals in the granule cell layer selectively innervated parvalbumin-containing cells, with numerous contacts per cell, whereas the granule cells were avoided. Under the electron microscope, those boutons made asymmetrical contacts on dendrites and somata. These findings suggest that, in addition to the hilar perforant path-associated (HIPP) interneurons, the basket and chandelier cells also receive a powerful feed-back drive from the granule cells, and thereby are able to control population synchrony in the dentate gyrus. On the other hand, the amount of monosynaptic excitatory feed-back among granule cells is shown to be negligible.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Coloring Agents
  • Dentate Gyrus / metabolism*
  • Dentate Gyrus / ultrastructure
  • Feedback / physiology*
  • Interneurons / metabolism*
  • Interneurons / ultrastructure
  • Male
  • Mossy Fibers, Hippocampal / metabolism*
  • Mossy Fibers, Hippocampal / ultrastructure
  • Neural Pathways / metabolism*
  • Neural Pathways / ultrastructure
  • Parvalbumins / metabolism*
  • Rats
  • Rats, Wistar
  • Synaptic Membranes / metabolism
  • Synaptic Membranes / ultrastructure
  • Zinc / metabolism


  • Coloring Agents
  • Parvalbumins
  • Zinc