Synapto-pHluorins: chimeras between pH-sensitive mutants of green fluorescent protein and synaptic vesicle membrane proteins as reporters of neurotransmitter release

Methods Enzymol. 2000;327:522-46. doi: 10.1016/s0076-6879(00)27300-x.
No abstract available

MeSH terms

  • Animals
  • Brain / metabolism
  • Cell Line
  • Cell Membrane / metabolism*
  • Gene Transfer Techniques
  • Green Fluorescent Proteins
  • Herpesvirus 1, Human / metabolism
  • Hippocampus / cytology
  • Humans
  • Hydrogen-Ion Concentration
  • Luminescent Proteins / chemistry*
  • Luminescent Proteins / metabolism*
  • Microscopy, Fluorescence / instrumentation*
  • Microscopy, Fluorescence / methods*
  • Neurons / metabolism
  • Neurotransmitter Agents / metabolism*
  • Rats
  • Recombinant Fusion Proteins / chemistry*
  • Recombinant Fusion Proteins / metabolism*
  • Synapses / metabolism*

Substances

  • Luminescent Proteins
  • Neurotransmitter Agents
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins