Between September 1996 and May 1999, the incidence and distribution of the main human rotavirus G genotypes (VP7 associated: G1-G4) and P genotypes (VP4 associated: P, P, P and P) among children with rotavirus gastroenteritis were determined using reverse transcription and polymerase chain reaction (RT-PCR)-based genotyping methods. From a total of 145 rotavirus strains examined, we identified the G type in 131 (90.3%) and the P type in 127 (87.5%) of the samples. An overall predominance of genotypes P G1 (42.7%) and P G4 (32.4%) was found during the period of study, with much lower incidence of genotypes P G2 (5.5%) and P G3 (2%). P and P types were not detected, neither were unusual combinations of P and G types. A significant genotypic shift was observed: whereas P G4 was the most prevalent genotype during the first year of the study (60%), the genotype P G1 gradually increased to account for 62.3% of the strains analysed in the following winter season. Mixed G types revealing dual infections G1/G4 and G3/G4 were found at low frequency (2%).