VP7 and VP4 genotypes among rotavirus strains recovered from children with gastroenteritis over a 3-year period in Valencia, Spain

Eur J Epidemiol. 2000 Jun;16(6):501-6. doi: 10.1023/a:1007618215377.


Between September 1996 and May 1999, the incidence and distribution of the main human rotavirus G genotypes (VP7 associated: G1-G4) and P genotypes (VP4 associated: P[8], P[4], P[6] and P[9]) among children with rotavirus gastroenteritis were determined using reverse transcription and polymerase chain reaction (RT-PCR)-based genotyping methods. From a total of 145 rotavirus strains examined, we identified the G type in 131 (90.3%) and the P type in 127 (87.5%) of the samples. An overall predominance of genotypes P[8] G1 (42.7%) and P[8] G4 (32.4%) was found during the period of study, with much lower incidence of genotypes P[4] G2 (5.5%) and P[8] G3 (2%). P[6] and P[9] types were not detected, neither were unusual combinations of P and G types. A significant genotypic shift was observed: whereas P[8] G4 was the most prevalent genotype during the first year of the study (60%), the genotype P[8] G1 gradually increased to account for 62.3% of the strains analysed in the following winter season. Mixed G types revealing dual infections G1/G4 and G3/G4 were found at low frequency (2%).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Antigens, Viral*
  • Capsid / genetics*
  • Capsid Proteins*
  • Child, Preschool
  • Electrophoresis, Polyacrylamide Gel
  • Feces / virology
  • Gastroenteritis / epidemiology
  • Gastroenteritis / virology*
  • Genotype
  • Humans
  • Infant
  • Infant, Newborn
  • Prospective Studies
  • RNA, Viral / analysis
  • Retrospective Studies
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rotavirus / classification
  • Rotavirus / genetics*
  • Rotavirus / isolation & purification
  • Rotavirus Infections / epidemiology
  • Rotavirus Infections / virology*
  • Spain / epidemiology


  • Antigens, Viral
  • Capsid Proteins
  • RNA, Viral
  • VP4 protein, Rotavirus
  • VP7 protein, Rotavirus