Analysing uncharted transcriptomes with SAGE

Trends Genet. 2000 Oct;16(10):423-5. doi: 10.1016/s0168-9525(00)02114-4.


Methods of comprehensive gene expression analysis have traditionally been limited to analysing bulk tissue or millions of cells. New modifications of serial analysis of gene expression (SAGE) have now permitted the analysis of gene expression in cell subpopulations or microanatomic structures, providing access to unexplored transcriptomes of normal and disease biology.

Publication types

  • Review

MeSH terms

  • Animals
  • DNA, Complementary / biosynthesis
  • DNA, Complementary / genetics*
  • DNA, Neoplasm / genetics
  • Databases, Factual
  • Expressed Sequence Tags*
  • Gene Expression Profiling*
  • Humans
  • Internet
  • Microchemistry / methods
  • Moloney murine leukemia virus / enzymology
  • Neoplasms / genetics
  • Polymerase Chain Reaction
  • RNA-Directed DNA Polymerase / metabolism
  • Sequence Homology, Nucleic Acid*
  • Transcription, Genetic*


  • DNA, Complementary
  • DNA, Neoplasm
  • RNA-Directed DNA Polymerase