Expression of cell volume-regulated kinase h-sgk in pancreatic tissue

Am J Physiol Gastrointest Liver Physiol. 2000 Nov;279(5):G998-G1002. doi: 10.1152/ajpgi.2000.279.5.G998.

Abstract

Transcript levels of the human serine/threonine kinase h-sgk have been found to be highest in pancreas. In the present study, localization and regulation of h-sgk transcription in pancreatic tissue were elucidated. As was apparent from radioactive in situ hybridization, most pancreatic acinar cells expressed high levels of h-sgk mRNA. h-sgk mRNA-positive cells were also found in ductal epithelia but not in pancreatic islets. In biopsy specimens from patients with pancreatitis, h-sgk mRNA levels were decreased in acinar cells but abundant in numerous mononuclear interstitial cells within areas of pancreatic necrosis and fibrosis. As shown by Northern blotting, h-sgk transcription in DAN-G pancreatic tumor cells is upregulated by osmotic cell shrinkage, serum, phorbol esters (phorbol 12,13-didecanoate), and Ca(2+) ionophore A-23187 and decreased by staurosporine and cAMP. In conclusion, h-sgk transcription is regulated not only by cell volume but also by serum, protein kinase C stimulation, cAMP, and increase of intracellular Ca(2+) activity. The kinase may participate not only in normal function of exocrine pancreas but also in fibrosing pancreatitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Blotting, Northern
  • Cell Size / physiology
  • Chronic Disease
  • Gene Expression Regulation, Enzymologic
  • Humans
  • Immediate-Early Proteins
  • In Situ Hybridization
  • Macrophages / physiology
  • Nuclear Proteins*
  • Pancreatic Ducts / cytology*
  • Pancreatic Ducts / enzymology*
  • Pancreatic Neoplasms
  • Pancreatitis / metabolism
  • Pancreatitis / pathology
  • Protein Serine-Threonine Kinases / genetics*
  • Protein Serine-Threonine Kinases / metabolism
  • RNA, Messenger / analysis
  • Transcriptional Activation / physiology
  • Tumor Cells, Cultured

Substances

  • Immediate-Early Proteins
  • Nuclear Proteins
  • RNA, Messenger
  • Protein Serine-Threonine Kinases
  • serum-glucocorticoid regulated kinase