Crystallographic studies of the interaction between the ferredoxin-NADP+ reductase and ferredoxin from the cyanobacterium Anabaena: looking for the elusive ferredoxin molecule

Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1408-12. doi: 10.1107/s0907444900010052.


Ferredoxin-NADP(+) reductase (FNR) and its physiological electron donor ferredoxin (Fd) from the cyanobacterium Anabaena PCC7119 have been co-crystallized. The unit-cell parameters are a = b = 63.72, c = 158.02 A and the space group is P2(1)2(1)2(1). The crystal structure has been solved with 2.4 A resolution synchrotron data by molecular replacement, anomalous dispersion and R(min) search methods. For the computations, the crystal was treated as a merohedral twin. The asymmetric unit contains two FNR molecules and one ferredoxin molecule. The packing of the FNR molecules displays a nearly tetragonal symmetry (space group P4(3)2(1)2), whereas the ferredoxin arrangement is orthorhombic. This study provides the first crystallographic model of a dissociable complex between FNR and Fd.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anabaena / chemistry*
  • Crystallography, X-Ray
  • Ferredoxin-NADP Reductase / chemistry*
  • Ferredoxins / chemistry*
  • Models, Molecular


  • Ferredoxins
  • Ferredoxin-NADP Reductase