Purification, crystallization and preliminary X-ray crystallographic analysis of human CCG1-interacting factor B

Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1479-81. doi: 10.1107/s0907444900010957.


A novel human factor CIB (CCG1-interacting factor B) has been isolated using the yeast two-hybrid system. The 22 kDa CIB protein has been expressed in Escherichia coli, purified to homogeneity and crystallized in a form suitable for crystallographic studies. The protein was crystallized in the orthogonal space group P2(1)2(1)2(1), with unit-cell parameters a = 43.60 (2), b = 44.45 (1), c = 110.70 (5) A and one molecule in the asymmetric unit. The crystal diffracted beyond 2.2 A resolution using synchrotron radiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle Proteins / chemistry*
  • Crystallography
  • Crystallography, X-Ray
  • Humans
  • Open Reading Frames
  • Protein Conformation


  • Cell Cycle Proteins