Kurosu, a traditional vinegar produced from unpolished rice, suppresses lipid peroxidation in vitro and in mouse skin

Biosci Biotechnol Biochem. 2000 Sep;64(9):1909-14. doi: 10.1271/bbb.64.1909.

Abstract

The in vitro antioxidative activities of various kinds of vinegar were investigated by using a linoleic acid autoxidation model detected by the thiobarbituric acid (TBA) method and the 1,1-diphenyl-2-picrylhydrazyl radical system. An ethyl acetate extract of Kurosu (EK), a vinegar made from unpolished rice, exhibited the highest antioxidative activity in both systems. EK (5 mg) inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced edema formation (14%) and myeloperoxidase activity (52%, P< 0.01) in female ICR mouse skin. Furthermore, EK significantly suppressed double TPA application-induced H2O2 generation (53%, P< 0.01) and lipid peroxidation determined by the TBA-reacting substance level (95 %, P< 0.01). In a two-stage carcinogenesis experiment with dimethylbenz[a]anthracene/TPA, EK significantly reduced the number of tumors per mouse by 36% (P<0.05) at 15 weeks after promotion. These results suggest that the antitumor-promoting effect may be partially due to the antioxidative properties of EK such as the decomposition of free radicals and interference with free radical-generating leukocytes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 9,10-Dimethyl-1,2-benzanthracene
  • Acetic Acid / analysis
  • Acetic Acid / pharmacology*
  • Animals
  • Antioxidants / pharmacology
  • Bepridil / analogs & derivatives
  • Bepridil / pharmacology
  • Biphenyl Compounds
  • Edema / chemically induced
  • Edema / physiopathology
  • Edema / prevention & control
  • Female
  • Free Radical Scavengers / pharmacology
  • Free Radicals / metabolism
  • Hydrogen Peroxide / metabolism
  • Inflammation / prevention & control
  • Lipid Peroxidation / drug effects*
  • Mice
  • Mice, Inbred ICR
  • Oryza*
  • Picrates*
  • Skin / drug effects*
  • Skin / metabolism
  • Skin / pathology
  • Skin Neoplasms / chemically induced
  • Skin Neoplasms / prevention & control
  • Tetradecanoylphorbol Acetate
  • Thiobarbituric Acid Reactive Substances / analysis
  • Time Factors
  • Vitamin E / pharmacology

Substances

  • Antioxidants
  • Biphenyl Compounds
  • Free Radical Scavengers
  • Free Radicals
  • Picrates
  • Thiobarbituric Acid Reactive Substances
  • Vitamin E
  • 9,10-Dimethyl-1,2-benzanthracene
  • Bepridil
  • Hydrogen Peroxide
  • 1,1-diphenyl-2-picrylhydrazyl
  • Tetradecanoylphorbol Acetate
  • Acetic Acid