Elevation of serum soluble tumour necrosis factor receptors in patients with polymyositis and dermatomyositis

Clin Rheumatol. 2000;19(5):352-9. doi: 10.1007/s100670070027.


The aim of the study was, to examine the relationship between serum levels of soluble tumour necrosis factor receptors (sTNF-R) and the gene expression of two types of receptor for TNF (TNF-R), a 55 a receptor (TNF-R1) and a 75 kDa receptor (TNF-R2), bloodin peripheral mononuclear cells (PBMC) from patients with polymyositis and dermatomyositis (PM/DM). Soluble tumour necrosis factor receptor 1 (sTNF-R1) and soluble tumour necrosis factor receptor 2 (sTNF-R2) levels in sera from patients were measured by enzyme-linked immunosorbent assay. Expression of TNF-R1 and TNF-R2 mRNAs in PBMC was analysed by Northern blotting. Serum sTNF-R1 and sTNF-R2 levels were elevated significantly in 25 patients with active-stage PM/DM, compared to those in 18 patients with inactive-stage PM/DM and 32 normal controls. Serum concentrations of sTNF-R1 and sTNF-R2 were correlated with PM/DM disease activity. TNF-R1 gene expression was enhanced in freshly isolated PBMC from patients with active-stage PM/DM. In contrast, TNF-R2 mRNA was expressed constitutively in patients with active-stage PM/DM and in normal controls. The expression of TNF-R1 and TNF-R2 mRNAs in PBMC and elevation of their soluble forms in the sera of patients with active-stage PM/DM suggest increased proteolytic cleavage of cell surface TNF-R from PBMC in patients with active-stage PM/DM, and that sTNF-R may regulate TNF-alpha-mediated muscle fibre damage in PM/DM.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Biomarkers / blood
  • Blotting, Northern
  • Chronic Disease
  • Creatine Kinase / metabolism
  • Dermatomyositis / blood
  • Disease Progression
  • Enzyme-Linked Immunosorbent Assay
  • Etanercept
  • Female
  • Fructose-Bisphosphate Aldolase / blood
  • Humans
  • Immunoglobulin G / blood*
  • Immunoglobulin G / genetics
  • Leukocytes, Mononuclear / metabolism
  • Longitudinal Studies
  • Male
  • Middle Aged
  • Polymyositis / blood*
  • RNA, Messenger / metabolism
  • Receptors, Tumor Necrosis Factor / blood*
  • Receptors, Tumor Necrosis Factor / genetics
  • Tumor Necrosis Factor-alpha / metabolism


  • Biomarkers
  • Immunoglobulin G
  • RNA, Messenger
  • Receptors, Tumor Necrosis Factor
  • Tumor Necrosis Factor-alpha
  • Creatine Kinase
  • Fructose-Bisphosphate Aldolase
  • Etanercept