Cloning of TC-1 (C8orf4), a novel gene found to be overexpressed in thyroid cancer

Genomics. 2000 Nov 1;69(3):342-7. doi: 10.1006/geno.2000.6348.


A novel gene highly expressed in thyroid cancer, designated TC-1 (thyroid cancer-1), was cloned from suppression subtractive hybridization between papillary thyroid carcinoma and its surrounding normal thyroid tissue. Overexpression of TC-1 in thyroid cancer was confirmed in 15/16 paired samples by RT-PCR and Northern analysis. Ubiquitously expressed in human tissues, the TC-1 sequence showed no homology to any known gene, but matched a cluster of ESTs. After alignment of our sequence with the ESTs, the missing transcription start site was obtained by 5'-RACE and verified by primer extension analysis. The full-length mRNA sequence of 1327 bp has an open reading frame of 321 bp, which encodes a highly conserved protein. Three regulatory motifs were identified at the expected positions within 1 kb of the 5' flanking sequence obtained by genome walking. Using fluorescence in situ hybridization, TC-1 was localized to chromosome 8p11.2. The overexpression of TC-1 in papillary carcinoma suggests that it may have an important role in thyroid carcinogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Animals
  • Base Sequence
  • Cattle
  • Chickens
  • Chromosome Mapping / methods
  • Chromosomes, Human, Pair 8*
  • Cloning, Molecular
  • DNA, Complementary
  • Exons
  • Gene Expression
  • Humans
  • Introns
  • Mice
  • Molecular Sequence Data
  • Neoplasm Proteins / classification
  • Neoplasm Proteins / genetics*
  • RNA, Messenger
  • Sequence Analysis, Protein / methods
  • Sequence Homology, Amino Acid
  • Thyroid Neoplasms / genetics*


  • Amino Acids
  • DNA, Complementary
  • Neoplasm Proteins
  • RNA, Messenger
  • TCIM protein, human

Associated data

  • GENBANK/AF268037
  • GENBANK/AF268038