Olive phenol hydroxytyrosol prevents passive smoking-induced oxidative stress

Circulation. 2000 Oct 31;102(18):2169-71. doi: 10.1161/01.cir.102.18.2169.


Background: Oxidative stress is involved in the onset of several degenerative disorders, and epidemiological studies indicate that a high intake of dietary antioxidants, as in the case of the Mediterranean basin, is protective. Olive mill waste waters (OMWWs) are a byproduct of olive oil production rich in phenolic antioxidants, such as hydroxytyrosol. We tested the effects of a low dose of an OMWW extract in a model of sidestream smoke-induced oxidative stress in rats by evaluating the urinary excretion of 8-iso-prostaglandin (PG) F(2alpha) (iPF(2alpha)-III).

Methods and results: An OMWW extract (5 mg/kg, providing 414 microg/kg of hydroxytyrosol) was administered to rats daily for 4 days, during which time the animals were exposed to sidestream smoke for 20 minutes once a day. Daily urines were collected, and the urinary excretion of 8-iso-PGF(2alpha) was evaluated as an index of oxidative stress-induced in vivo lipid peroxidation. The exposure of rats to passive smoking increased the urinary excretion of 8-iso-PGF(2alpha) by 44+/-4.2% at 48 hours and by 55+/-10% at 96 hours. Treatment with the OMWW extract was able to completely prevent the increase at 48 hours and resulted in lower increments (34+/-18% versus 55+/-10%) of 8-iso-PGF(2alpha) excretion at 96 hours.

Conclusions: A low dose of hydroxytyrosol, administered through OMWW, reduces the consequences of sidestream smoke-induced oxidative stress in rats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Creatinine / urine
  • Dinoprost / analogs & derivatives*
  • Dinoprost / urine
  • F2-Isoprostanes
  • Inhalation Exposure
  • Lipid Peroxidation / drug effects
  • Male
  • Oxidative Stress / drug effects*
  • Phenylethyl Alcohol / analogs & derivatives*
  • Phenylethyl Alcohol / pharmacology*
  • Rats
  • Rats, Sprague-Dawley
  • Tobacco Smoke Pollution / adverse effects*
  • Trees / chemistry


  • Antioxidants
  • F2-Isoprostanes
  • Tobacco Smoke Pollution
  • 3,4-dihydroxyphenylethanol
  • 8-epi-prostaglandin F2alpha
  • Creatinine
  • Dinoprost
  • Phenylethyl Alcohol