Abstract
MAT1 (ménage à trois1) functions as an assembly/targeting factor of CAK (cyclin-dependent kinase-activating kinase). In a search for MAT1-interacting proteins using yeast two-hybrid system, MCM7 (minichromosome maintenance 7), a member of a family of DNA licensing factors, was identified. The physical interaction between MAT1 and MCM7 was confirmed in vivo in yeast cells and verified with in vitro protein binding assays. Further studies showed the RING-finger motif of MAT1 is not required for the interaction with MCM7, while the C-terminal domain of MAT1 is indispensable. Immunoprecipitation of MCM7 in human osteosarcoma MG63 cells demonstrated that MCM7 associates with the CAK complex in vivo.
MeSH terms
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Amino Acid Motifs
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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Cyclin H
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Cyclin-Dependent Kinase-Activating Kinase
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Cyclin-Dependent Kinases*
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Cyclins / metabolism
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Humans
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Macromolecular Substances
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Minichromosome Maintenance Complex Component 7
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Precipitin Tests
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Protein Binding
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Protein Serine-Threonine Kinases / chemistry
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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Protein Structure, Tertiary
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Substrate Specificity
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Tumor Cells, Cultured
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Two-Hybrid System Techniques
Substances
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CCNH protein, human
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Cell Cycle Proteins
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Cyclin H
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Cyclins
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DNA-Binding Proteins
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Macromolecular Substances
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Nuclear Proteins
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Protein Serine-Threonine Kinases
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Cyclin-Dependent Kinases
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MCM7 protein, human
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Minichromosome Maintenance Complex Component 7
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Cyclin-Dependent Kinase-Activating Kinase