Column-switching HPLC for the analysis of plasma in PET imaging studies

Nucl Med Biol. 2000 Aug;27(6):627-30. doi: 10.1016/s0969-8051(00)00125-6.

Abstract

A column-switch high performance liquid chromatography method for the analysis of 4 mL of plasma is described with six examples of chromatography of [(11)C]-labeled positron-emission tomography imaging agents. Complete extraction of all but the most polar metabolites by the reverse phase capture column is achieved by disruption of plasma protein binding by 8 M urea.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromatography, High Pressure Liquid / instrumentation
  • Chromatography, High Pressure Liquid / methods*
  • Cocaine / analogs & derivatives*
  • Cocaine / blood
  • Dopamine Agents / blood
  • Imidazoles / blood
  • Isoquinolines / blood
  • Ligands
  • Pyridines / blood
  • Raclopride / blood
  • Sensitivity and Specificity
  • Solvents / chemistry
  • Tetrabenazine / analogs & derivatives
  • Tetrabenazine / blood
  • Time Factors
  • Tomography, Emission-Computed*
  • Urea / chemistry

Substances

  • Dopamine Agents
  • Imidazoles
  • Isoquinolines
  • Ligands
  • Pyridines
  • Solvents
  • L 159884
  • dihydrotetrabenazine
  • Raclopride
  • (1R-(exo,exo))-3-(4-fluorophenyl)-8-methyl-8- azabicyclo(3.2.1)octane-2-carboxylic acid, methyl ester
  • Urea
  • McN 5652
  • Cocaine
  • Tetrabenazine