Novel signaling from the peripodial membrane is essential for eye disc patterning in Drosophila

Cell. 2000 Oct 13;103(2):331-42. doi: 10.1016/s0092-8674(00)00124-0.

Abstract

The Drosophila eye disc is a sac of single layer epithelium with two opposing sides, the peripodial membrane (PM) and the disc proper (DP). Retinal morphogenesis is organized by Notch signaling at the dorsoventral (DV) boundary in the DP. Functions of the PM in coordinating growth and patterning of the DP are unknown. We show that the secreted proteins, Hedgehog, Wingless, and Decapentaplegic, are expressed in the PM, yet they control DP expression of Notch ligands, Delta and Serrate. Peripodial clones expressing Hedgehog induce Serrate in the DP while loss of peripodial Hedgehog disrupts disc growth. Furthermore, PM cells extend cellular processes to the DP. Therefore, peripodial signaling is critical for eye pattern formation and may be mediated by peripodial processes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Body Patterning*
  • Calcium-Binding Proteins
  • Drosophila / embryology*
  • Drosophila Proteins*
  • Embryonic Induction*
  • Eye / embryology*
  • Gene Expression Regulation, Developmental
  • Head / embryology
  • Hedgehog Proteins
  • Insect Proteins / metabolism
  • Intercellular Signaling Peptides and Proteins
  • Jagged-1 Protein
  • Larva
  • Membrane Proteins / metabolism
  • Nuclear Proteins / metabolism
  • Phosphoproteins / metabolism
  • Photoreceptor Cells, Invertebrate / embryology
  • Proto-Oncogene Proteins / metabolism
  • Serrate-Jagged Proteins
  • Transcription Factors*
  • Wnt1 Protein

Substances

  • Calcium-Binding Proteins
  • Drosophila Proteins
  • Hedgehog Proteins
  • Insect Proteins
  • Intercellular Signaling Peptides and Proteins
  • Jagged-1 Protein
  • Membrane Proteins
  • Nuclear Proteins
  • Phosphoproteins
  • Proto-Oncogene Proteins
  • Ser protein, Drosophila
  • Serrate-Jagged Proteins
  • Transcription Factors
  • Wnt1 Protein
  • dl protein, Drosophila
  • dpp protein, Drosophila
  • wg protein, Drosophila
  • hh protein, Drosophila