Peripodial Cells Regulate Proliferation and Patterning of Drosophila Imaginal Discs

Cell. 2000 Oct 13;103(2):343-50. doi: 10.1016/s0092-8674(00)00125-2.

Abstract

Cells employ a diverse array of signaling mechanisms to establish spatial patterns during development. Nowhere is this better understood than in Drosophila, where the limbs and eyes arise from discrete epithelial sacs called imaginal discs. Molecular-genetic analyses of pattern formation have generally treated discs as single epithelial sheets. Anatomically, however, discs comprise a columnar cell monolayer covered by a squamous epithelium known as the peripodial membrane. Here we demonstrate that during development, peripodial cells signal to disc columnar cells via microtubule-based apical extensions. Ablation and targeted gene misexpression experiments demonstrate that peripodial cell signaling contributes to growth control and pattern formation in the eye and wing primordia. These findings challenge the traditional view of discs as monolayers and provide foundational evidence for peripodial cell function in Drosophila appendage development.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biological Transport
  • Body Patterning*
  • Cell Surface Extensions / metabolism
  • Drosophila / cytology
  • Drosophila / embryology*
  • Drosophila Proteins
  • Embryonic Induction*
  • Epithelial Cells*
  • Eye / anatomy & histology
  • Eye / embryology
  • Microtubule-Associated Proteins / metabolism
  • Microtubules / metabolism
  • Models, Biological
  • Molecular Motor Proteins / metabolism
  • Photoreceptor Cells, Invertebrate / anatomy & histology
  • Photoreceptor Cells, Invertebrate / embryology
  • Wings, Animal / embryology

Substances

  • DCTN1-p150 protein, Drosophila
  • Drosophila Proteins
  • Microtubule-Associated Proteins
  • Molecular Motor Proteins