Chemoenzymatic synthesis of L-galactosylated dimeric sialyl Lewis X structures employing alpha-1,3-fucosyltransferase V

Bioorg Med Chem. 2000 Oct;8(10):2519-25. doi: 10.1016/s0968-0896(00)00187-5.

Abstract

L-Galactosylated dimeric sialyl Lewis X (SLeX) has been prepared employing a combination of chemical and enzymatic synthetic methods. GDP-L-galactose has been chemically synthesised. Enzymatic transfer of L-galactose onto the acceptor (Sia-alpha2,3-Gal-beta1,4-GlcNAc-beta1,3/6)2-Man-alpha1-OMe was achieved using the human alpha-1,3-fucosyltransferase V.

MeSH terms

  • Carbohydrate Sequence
  • Chromatography, Thin Layer
  • Fucosyltransferases / chemistry*
  • Galactose
  • Glycosylation
  • Humans
  • Molecular Sequence Data
  • Oligosaccharides / chemical synthesis*
  • Sialyl Lewis X Antigen

Substances

  • Oligosaccharides
  • Sialyl Lewis X Antigen
  • Fucosyltransferases
  • Galactose