Polymorphisms of the DNA repair gene XRCC1 and risk of gastric cancer in a Chinese population

Int J Cancer. 2000 Nov 15;88(4):601-6. doi: 10.1002/1097-0215(20001115)88:4<601::aid-ijc13>3.0.co;2-c.

Abstract

Gastric cancer remains the leading cause of cancer death in China and other countries in eastern Asia. Studies of gastric cancer have revealed that it is a disease of complex etiology involving dietary, infectious, environmental, occupational and genetic factors. DNA repair capacity has been suggested as a genetic factor contributing to variation in susceptibility to cancer. In the present study, we described an association between 2 polymorphisms of the DNA repair gene XRCC1 and risk of gastric cancer in a Chinese population. We used a polymerase chain reaction-based assay to detect Pvu II and Nci I restriction fragment length polymorphisms (XRCC1 26304 C-->T and XRCC1 28152 G-->A, respectively) in 188 patients with gastric cancer and 166 healthy controls. The XRCC1 26304 T allele (194Trp) frequency (34.6%) was higher and the XRCC1 28152 A allele (399Gln) frequency (25.6%) was lower in healthy Chinese controls than previously reported healthy U.S. Caucasian controls (7.2% and 34.1%, respectively). Multivariate logistic regression analysis revealed that the putative high-risk genotypes XRCC1 26304 CC and XRCC1 28152 GA/AA were associated with a non-significant increased risk for gastric cancer (adjusted odds ratio [OR]=1.45, 95% confidence interval [CI]= 0.93-2.25 and OR=1.53, 95% CI= 0.98-2.39, respectively) compared with other genotypes. However, the XRCC1 26304 CC genotype was associated with a significantly increased risk for gastric cardia cancer (adjusted OR=1.86, 95% CI=1.09-3.20). Individuals with both putative high-risk genotypes (CC and GA/AA) had a significantly higher risk (adjusted OR=1.73, 95% CI=1.12-2.69), particularly for gastric cardia cancer (adjusted OR=2.18, 95% CI=1.21-3.94) than individuals with other genotypes. These findings support the hypothesis that these 2 XRCC1 variants may contribute to the risk of developing gastric cancer, particularly gastric cardia cancer.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Alcohol Drinking
  • Asian Continental Ancestry Group / genetics
  • China
  • DNA Repair*
  • DNA-Binding Proteins / genetics*
  • Deoxyribonucleases, Type II Site-Specific
  • Female
  • Gene Frequency
  • Helicobacter Infections / epidemiology
  • Humans
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Polymerase Chain Reaction / methods
  • Polymorphism, Genetic*
  • Polymorphism, Restriction Fragment Length*
  • Reference Values
  • Regression Analysis
  • Risk Factors
  • Smoking
  • Stomach Neoplasms / epidemiology*
  • Stomach Neoplasms / genetics*
  • X-ray Repair Cross Complementing Protein 1

Substances

  • DNA-Binding Proteins
  • X-ray Repair Cross Complementing Protein 1
  • XRCC1 protein, human
  • CAGCTG-specific type II deoxyribonucleases
  • CCSGG-specific type II deoxyribonucleases
  • Deoxyribonucleases, Type II Site-Specific