Fluorovisualization effect of acridine orange on mouse osteosarcoma

Anticancer Res. 2000 Sep-Oct;20(5A):3019-24.


If the localization of musculoskeletal sarcomas could be visualized during surgery, it would be possible to completely resect the tumor with minimum damage to normal tissues and the patients could retain a functional limb. Therefore, we conducted the present study to clarify the usefulness of acridine orange (AO) for fluorovisualization of tumors using a mouse osteosarcoma model. At 2 hours after injection of 10 mg/kg AO to mice inoculated with MOS mouse osteosarcoma cells, fluorovisualization of mouse osteosarcoma reached the maximum level. Even a 1-mm-diameter lesion of pulmonary metastasis was visualized. The results suggested that AO may be useful for specific fluorovisualization of human osteosarcomas during surgery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acridine Orange*
  • Animals
  • Bone Neoplasms / pathology*
  • Disease Models, Animal
  • Fluorescence
  • Fluorescent Dyes*
  • Humans
  • Male
  • Mice
  • Mice, Inbred C3H
  • Osteosarcoma / pathology*


  • Fluorescent Dyes
  • Acridine Orange