Establishment of a human malignant fibrous histiocytoma cell line, COMA. Characterization By conventional cytogenetics, comparative genomic hybridization, and multiplex fluorescence In situ hybridization

Cancer Genet Cytogenet. 2000 Sep;121(2):117-23. doi: 10.1016/s0165-4608(00)00261-2.


The human COMA cell line has been established from a storiform pleomorphic malignant fibrous histiocytoma (MFH). As expected for this tumor type, a very complex karyotype was observed after R-banding analysis. An extensive analysis by 24-color painting, comparative genomic hybridization (CGH), and fluorescence in situ hybridization (FISH) was performed. Twelve complex marker chromosomes recurrently observed were clearly identified; among them, three were systematically present in all analyzed metaphases. Amplifications detected by CGH were refined by FISH with probes specific for various candidate loci. A significant aneuploidy and numerous micronuclei were observed, which could be related to the anomalies of centriole numbers detected in a proportion of cells. Such an analysis, performed on a series of MFH cell lines, would allow the delineation of the genomic alterations specific for the oncogenesis or progression of this complex tumor type or both.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Centrosome
  • Chromosomes, Artificial, Yeast
  • Female
  • Histiocytoma, Benign Fibrous / genetics
  • Histiocytoma, Benign Fibrous / pathology*
  • Histiocytoma, Benign Fibrous / ultrastructure
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Karyotyping
  • Nucleic Acid Hybridization / methods*
  • Soft Tissue Neoplasms / genetics
  • Soft Tissue Neoplasms / pathology*
  • Soft Tissue Neoplasms / ultrastructure
  • Tumor Cells, Cultured