doi: 10.1042/bj1500013.
Synthesis time of beta-galactosidase in Escherichia coli B/r as a function of growth rate
- PMID: 1106403
- PMCID: PMC1165698
- DOI: 10.1042/bj1500013
Item in Clipboard
Synthesis time of beta-galactosidase in Escherichia coli B/r as a function of growth rate
Biochem J.
1975 Jul.
Abstract
By analysing the kinetics of beta-galactosidase accumulation after induction, the synthesis time of beta-galactosidase in Escherichia coli B/r was found to be 75s in rapidly growing cells (1.36 and 2.1 doublings/h), and 90s in slowly growing cells (0.63 doubling/h). These values correspond to peptide-chain-elongation rates of 16 and 13 amino acids/s respectively, in agreement with previous findings, indicating that the peptide-chain growth rate is constant (presumably maximal) in fast-growing bacteria, but decreased in slowly growing bacteria [Forchhammer & Lindahl (1971) J. Mol. Biol. 55, 563-568].
Similar articles
-
Polypeptide-chain-elongation rate in Escherichia coli B/r as a function of growth rate.Biochem J. 1976 Nov 15;160(2):185-94. doi: 10.1042/bj1600185. Biochem J. 1976. PMID: 795428 Free PMC article.
-
Metabolic regulation of beta-galactosidase synthesis in Escherichia coli. A test for constitutive ribosome synthesis.Biochem J. 1975 Jul;150(1):1-8. doi: 10.1042/bj1500001b. Biochem J. 1975. PMID: 173297 Free PMC article.
-
The synthesis of beta-galactosidase by constitutive and other regulatory mutants of Escherichia coli in chemostat culture.J Gen Microbiol. 1975 Aug;89(2):221-8. doi: 10.1099/00221287-89-2-221. J Gen Microbiol. 1975. PMID: 170362
-
Chain elongation rate of messenger and polypeptides in slowly growing Escherichia coli.J Mol Biol. 1971 Aug 28;60(1):1-19. doi: 10.1016/0022-2836(71)90442-6. J Mol Biol. 1971. PMID: 4937191 No abstract available.
-
Induction kinetics of the L-arabinose operon of Escherichia coli.J Bacteriol. 1973 Jul;115(1):9-14. doi: 10.1128/jb.115.1.9-14.1973. J Bacteriol. 1973. PMID: 4577756 Free PMC article.
Cited by
-
The functional stability of the lacZ transcript is sensitive towards sequence alterations immediately downstream of the ribosome binding site.Mol Gen Genet. 1987 Aug;209(1):179-87. doi: 10.1007/BF00329856. Mol Gen Genet. 1987. PMID: 3312955
-
Escherichia coli ribosomes translate in vivo with variable rate.EMBO J. 1984 Dec 1;3(12):2895-8. doi: 10.1002/j.1460-2075.1984.tb02227.x. EMBO J. 1984. PMID: 6396082 Free PMC article.
-
Polypeptide-chain-elongation rate in Escherichia coli B/r as a function of growth rate.Biochem J. 1976 Nov 15;160(2):185-94. doi: 10.1042/bj1600185. Biochem J. 1976. PMID: 795428 Free PMC article.
-
Real time determination of bacterial in vivo ribosome translation elongation speed based on LacZα complementation system.Nucleic Acids Res. 2016 Nov 16;44(20):e155. doi: 10.1093/nar/gkw698. Epub 2016 Oct 5. Nucleic Acids Res. 2016. PMID: 27903884 Free PMC article.
-
Reduction of translating ribosomes enables Escherichia coli to maintain elongation rates during slow growth.Nat Microbiol. 2016 Dec 12;2:16231. doi: 10.1038/nmicrobiol.2016.231. Nat Microbiol. 2016. PMID: 27941827 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
