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. 2000 Nov;31(5):390-4.
doi: 10.1046/j.1472-765x.2000.00834.x.

An ELISA for the detection of Bacillus subtilis L-form bacteria confirms their symbiosis in strawberry

C M Ferguson  1 , N A Booth, E J Allan
Affiliations

An ELISA for the detection of Bacillus subtilis L-form bacteria confirms their symbiosis in strawberry

C M Ferguson et al. Lett Appl Microbiol. 2000 Nov.

Abstract

Aims: To develop an ELISA for the detection of antigens derived from stable Bacillus subtilis L-form bacteria and to detect these in plants injected with L-form bacteria.

Methods and results: A sandwich ELISA was developed and its specificity was investigated using L-forms and cell-walled forms of B. subtilis, different Bacillus species and a range of bacteria isolated from glasshouse-grown strawberry plants. The detection limits of the ELISA were approximately 10(3) viable cells ml(-1) for L-forms compared with 10(7) viable cells ml(-1) for cell-walled forms. Results showed that L-forms survived and moved within strawberry tissues injected with L-form bacteria.

Conclusion: An ELISA that selectively detects B. subtilis L-form bacteria was developed and shown to confirm the presence of L-forms in plants.

Significance and impact of the study: This will be a valuable rapid method to further studies on L-form plant interactions.

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