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, 13 (4), 453-62

CD40 Triggering of Heterodimeric IL-12 p70 Production by Dendritic Cells in Vivo Requires a Microbial Priming Signal

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CD40 Triggering of Heterodimeric IL-12 p70 Production by Dendritic Cells in Vivo Requires a Microbial Priming Signal

O Schulz et al. Immunity.

Abstract

CD40 ligation triggers IL-12 production by dendritic cells (DC) in vitro. Here, we demonstrate that CD40 cross-linking alone is not sufficient to induce IL-12 production by DC in vivo. Indeed, resting DC make neither the IL-12 p35 nor IL-12 p40 subunits and express only low levels of CD40. Nevertheless, after DC activation by microbial stimuli that primarily upregulate IL-12 p40 and augment CD40 expression, CD40 ligation induces a significant increase in IL-12 p35 and IL-12 p70 heterodimer production. Similarly, IL-12 p70 is produced during T cell activation in the presence but not in the absence of microbial stimuli. Thus, production of bioactive IL-12 by DC can be amplified by T cell-derived signals but must be initiated by innate signals.

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