EBV-infected B Cells in Infectious Mononucleosis: Viral Strategies for Spreading in the B Cell Compartment and Establishing Latency

Immunity. 2000 Oct;13(4):485-95. doi: 10.1016/s1074-7613(00)00048-0.

Abstract

Infection of humans with Epstein-Barr virus (EBV) may cause infectious mononucleosis (IM). Analysis of single EBV-infected cells from tonsils of IM patients for rearranged immunoglobulin genes revealed two strategies of EBV for rapid and massive spread in the B cell compartment: the direct infection of many naive as well as memory and/or germinal center B cells and the expansion of the latter cells to large clones. In IM, the generation of virus-harboring memory B cells from naive B cells passing through a germinal center reaction likely plays no role. Members of clones can show distinct morphologies and likely also EBV gene expression patterns, and this ability implies a mechanism by which EBV-harboring cells can evade immune surveillance and establish a pool of persisting EBV-infected B cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • B-Lymphocyte Subsets / immunology*
  • B-Lymphocyte Subsets / metabolism
  • B-Lymphocyte Subsets / pathology
  • B-Lymphocyte Subsets / virology*
  • Cell Differentiation / genetics
  • Cell Differentiation / immunology
  • Clone Cells
  • Female
  • Gene Expression Regulation, Viral / immunology
  • Gene Rearrangement, B-Lymphocyte
  • Genetic Variation / immunology
  • Germinal Center / immunology
  • Germinal Center / pathology
  • Germinal Center / virology
  • Herpesvirus 4, Human / growth & development
  • Herpesvirus 4, Human / immunology*
  • Herpesvirus 4, Human / isolation & purification
  • Herpesvirus 4, Human / pathogenicity
  • Hodgkin Disease / immunology
  • Hodgkin Disease / pathology
  • Hodgkin Disease / virology
  • Humans
  • Immunologic Memory
  • Immunophenotyping
  • Infectious Mononucleosis / immunology*
  • Infectious Mononucleosis / pathology
  • Infectious Mononucleosis / virology*
  • Interphase / immunology
  • Male
  • Molecular Sequence Data
  • Organ Specificity / immunology
  • Palatine Tonsil / immunology
  • Palatine Tonsil / pathology
  • Palatine Tonsil / virology
  • Polymerase Chain Reaction
  • Reed-Sternberg Cells / immunology
  • Reed-Sternberg Cells / pathology
  • Reed-Sternberg Cells / virology
  • Virus Latency / immunology*
  • Virus Replication / immunology*

Associated data

  • GENBANK/AJ275352
  • GENBANK/AJ275353
  • GENBANK/AJ275354
  • GENBANK/AJ275355
  • GENBANK/AJ275356
  • GENBANK/AJ275357
  • GENBANK/AJ275358
  • GENBANK/AJ275359
  • GENBANK/AJ275360
  • GENBANK/AJ275361
  • GENBANK/AJ275362
  • GENBANK/AJ275363
  • GENBANK/AJ275364
  • GENBANK/AJ275365
  • GENBANK/AJ275366
  • GENBANK/AJ275367
  • GENBANK/AJ275368
  • GENBANK/AJ275369
  • GENBANK/AJ275370
  • GENBANK/AJ275371
  • GENBANK/AJ275372
  • GENBANK/AJ275373
  • GENBANK/AJ275374
  • GENBANK/AJ275375
  • GENBANK/AJ275376
  • GENBANK/AJ275377
  • GENBANK/AJ275378
  • GENBANK/AJ275379
  • GENBANK/AJ275380
  • GENBANK/AJ275381