Role of nitric oxide in adrenocorticotrophin-induced hypertension: L-arginine effects reversed by N-nitro-L-arginine

Clin Exp Pharmacol Physiol. 2000 Nov;27(11):887-90. doi: 10.1046/j.1440-1681.2000.03354.x.

Abstract

1. L-arginine prevents adrenocorticotrophin (ACTH)-induced hypertension in the rat. To confirm that this effect is mediated through the nitric oxide (NO) system, we examined whether N-nitro-L-arginine (NOLA) could reverse the L-arginine-induced blockade of ACTH-induced hypertension. 2. Blood pressure and metabolic parameters were examined in sham-, ACTH-, L-arginine + sham-, NOLA + sham-, ACTH + L-arginine- and ACTH + L-arginine + NOLA-treated Sprague-Dawley rats (n = 40). 3. Adrenocorticotrophin treatment increased systolic blood pressure (SBP), water intake and urine output and decreased bodyweight. N-Nitro-L-arginine alone increased SBP without affecting metabolic variables. L-Arginine alone did not affect blood pressure. The SBP was lower in L-arginine + ACTH- than ACTH-treated rats (P < 0.001), but was higher following ACTH + L-arginine + NOLA than ACTH + L-arginine (P < 0.05). 4. N-Nitro-L-arginine reversed the blood pressure-lowering effect of L-arginine in ACTH-induced hypertension in the rat, supporting the notion that NO plays a role in the hypertension.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenocorticotropic Hormone / pharmacology*
  • Animals
  • Arginine / antagonists & inhibitors
  • Arginine / pharmacology*
  • Blood Pressure / drug effects
  • Drinking
  • Eating
  • Enzyme Inhibitors / pharmacology*
  • Hypertension / chemically induced*
  • Hypertension / physiopathology
  • Male
  • Nitric Oxide / physiology*
  • Nitric Oxide Synthase / antagonists & inhibitors*
  • Nitric Oxide Synthase Type III
  • Nitroarginine / pharmacology*
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Enzyme Inhibitors
  • Nitroarginine
  • Nitric Oxide
  • Adrenocorticotropic Hormone
  • Arginine
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type III
  • Nos3 protein, rat