Quantitative PCR analysis of different splice forms of NFAT5 revealed specific gene expression in fetal and adult brain

Brain Res Mol Brain Res. 2000 Nov 10;83(1-2):125-7. doi: 10.1016/s0169-328x(00)00202-3.


To investigate sequences or mutations associated with neurodegenerative disorders, we performed analyses for the NFAT5 gene, which is located in the candidate region for the autosomal dominantly inherited spinocerebellar ataxia type 4 (SCA4). PCR based expression analyses detected NFAT5 transcripts with alternative splicing in 27 fetal and adult human tissues. Interestingly, by using quantitative methods on cDNA from fetal and adult human brain a significant difference at the expression level for one splice form could be shown.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Alternative Splicing / genetics*
  • Brain Chemistry / genetics
  • DNA-Binding Proteins / genetics*
  • Exons
  • Fetus
  • Gene Expression Regulation, Developmental*
  • Humans
  • Introns
  • Molecular Sequence Data
  • NFATC Transcription Factors
  • Polymerase Chain Reaction / methods
  • RNA Splice Sites
  • Spinocerebellar Ataxias / genetics*
  • Transcription Factors / genetics*


  • DNA-Binding Proteins
  • NFATC Transcription Factors
  • RNA Splice Sites
  • Transcription Factors

Associated data

  • GENBANK/AJ005683
  • GENBANK/AJ005684
  • GENBANK/AJ243299
  • GENBANK/AJ243980