p53-mediated negative regulation of stathmin/Op18 expression is associated with G(2)/M cell-cycle arrest

Int J Cancer. 2000 Dec 1;88(5):685-91. doi: 10.1002/1097-0215(20001201)88:5<685::aid-ijc1>3.0.co;2-z.


Utilizing the technique of differential display of mRNA, we have identified p53-responsive genes that are transcriptionally up- or down-regulated as cells enter growth arrest. One gene that was down-regulated, pong16, was found to be identical to stathmin/Op18, a protein involved in the regulation of microtubule dynamics. Evidence that p53 is directly or indirectly involved in negative regulation of stathmin/Op18 expression includes the following: (i) p53-mediated growth inhibition is associated with repression of stathmin/Op18 expression following serum stimulation, (ii) reporter gene assays revealed p53-mediated repression of stathmin/Op18 promoter activity and (iii) constitutive over-expression of stathmin/Op18 bypasses a p53-mediated G(2)/M arrest in the cell cycle. These results suggest that p53-mediated negative regulation of stathmin/Op18 plays an important role in cell-cycle control.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Down-Regulation
  • G2 Phase / physiology*
  • Gene Expression Regulation*
  • Humans
  • Microtubule Proteins*
  • Mitosis / physiology*
  • Phosphoproteins / biosynthesis
  • Phosphoproteins / genetics*
  • Promoter Regions, Genetic / physiology
  • RNA, Messenger / biosynthesis
  • Stathmin
  • Tumor Cells, Cultured
  • Tumor Suppressor Protein p53 / physiology*


  • Microtubule Proteins
  • Phosphoproteins
  • RNA, Messenger
  • STMN1 protein, human
  • Stathmin
  • Tumor Suppressor Protein p53