Nuclear transfer in cattle : effect of linoleic acid-albumin on freezing sensitivity of enucleated oocytes

J Vet Med Sci. 2000 Oct;62(10):1111-3. doi: 10.1292/jvms.62.1111.

Abstract

The effect of linoleic acid-albumin (LAA) supplementation to the media for IVM, enucleation, and activation on the developmental potential of bovine embryos produced by nuclear transfer (NT) into frozen-thawed cytoplasts was investigated. Blastomeres derived from morulae was placed in the perivitelline space of frozen-thawed cytoplasts, which were then fused by a DC pulse. The proportion of fused embryos was similar between groups with and without LAA (87 vs. 90%). The proportion of development to blastocysts of NT embryos derived from the media with LAA (14%) was higher than that without LAA (4%), indicating that LAA treatment of bovine oocytes during IVM, enucleation and activation can improve the ability of such cytoplasts after freezing and thawing to develop into blastocysts after NT.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Albumins*
  • Animals
  • Cattle / physiology*
  • Cryopreservation / veterinary
  • Culture Media
  • Freezing
  • Gene Transfer Techniques / veterinary*
  • Linoleic Acid*
  • Oocytes / physiology*

Substances

  • Albumins
  • Culture Media
  • Linoleic Acid