Celloidin mounting (embedding without infiltration) - a new, simple and reliable method for producing serial sections of high thickness through complete human brains and its application to stereological and immunohistochemical investigations

J Chem Neuroanat. 2000 Oct;20(1):49-59. doi: 10.1016/s0891-0618(00)00067-3.

Abstract

Celloidin mounting (embedding without infiltration) of the human central nervous system (CNS) proved to be superior to gelatin embedding for the production of serial sections ranging in thickness from 220 to 500 microm. After gallocyanin-staining, a comprehensive neuroanatomical as well as neuropathological survey of the human brain is possible, including diagnosis of Alzheimer's disease. Details of a fractionator analysis of the total striatal neuron number are described and the possible quantitative analysis of parallel immunohistochemically stained sections is discussed.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Algorithms
  • Alzheimer Disease / pathology
  • Brain / anatomy & histology*
  • Cell Count
  • Collodion*
  • Desiccation
  • Female
  • Gelatin
  • Humans
  • Imaging, Three-Dimensional
  • Immunohistochemistry / methods*
  • Male
  • Microtomy
  • Middle Aged
  • Neurons / physiology
  • Plastic Embedding / methods*

Substances

  • Gelatin
  • Collodion