High-throughput screening for protein-protein interactions using two-hybrid assay

Methods Enzymol. 2000;328:3-14. doi: 10.1016/s0076-6879(00)28386-9.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cloning, Molecular / methods
  • DNA Primers
  • DNA-Binding Proteins
  • Fungal Proteins / biosynthesis
  • Fungal Proteins / genetics
  • Molecular Sequence Data
  • Open Reading Frames
  • Plasmids
  • Recombinant Fusion Proteins / biosynthesis*
  • Recombinant Fusion Proteins / chemistry
  • Restriction Mapping
  • Robotics
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics
  • Two-Hybrid System Techniques

Substances

  • DNA Primers
  • DNA-Binding Proteins
  • Fungal Proteins
  • GAL4 protein, S cerevisiae
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors