A bacterial two-hybrid system that exploits a cAMP signaling cascade in Escherichia coli

Methods Enzymol. 2000;328:59-73. doi: 10.1016/s0076-6879(00)28390-0.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylyl Cyclases / chemistry*
  • Adenylyl Cyclases / genetics*
  • Amino Acid Sequence
  • Bordetella pertussis / enzymology
  • Bordetella pertussis / genetics
  • Catalytic Domain
  • Cloning, Molecular / methods
  • Culture Media
  • Cyclic AMP / physiology*
  • Escherichia coli / genetics*
  • Genes, Reporter
  • Molecular Sequence Data
  • Mutagenesis
  • Point Mutation
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / chemistry
  • Signal Transduction / physiology*
  • Two-Hybrid System Techniques
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism

Substances

  • Culture Media
  • Recombinant Fusion Proteins
  • Cyclic AMP
  • beta-Galactosidase
  • Adenylyl Cyclases