Mapping specificity determinants for protein-protein association using protein fusions and random peptide libraries

Methods Enzymol. 2000;328:157-70. doi: 10.1016/s0076-6879(00)28397-3.
No abstract available

MeSH terms

  • Amino Acid Sequence
  • Binding Sites
  • Cloning, Molecular
  • Consensus Sequence
  • Electrophoresis, Polyacrylamide Gel / methods
  • Escherichia coli
  • Glutathione Transferase / genetics
  • Glutathione Transferase / isolation & purification
  • Molecular Sequence Data
  • Oligopeptides / chemical synthesis*
  • Oligopeptides / chemistry
  • Peptide Library*
  • Peptides / chemistry
  • Recombinant Fusion Proteins / chemistry*
  • Recombinant Fusion Proteins / isolation & purification*
  • Recombinant Fusion Proteins / metabolism

Substances

  • Oligopeptides
  • Peptide Library
  • Peptides
  • Recombinant Fusion Proteins
  • Glutathione Transferase