A systematic and general proteolytic method for defining structural and functional domains of proteins

Methods Enzymol. 2000;328:499-514. doi: 10.1016/s0076-6879(00)28415-2.
No abstract available

MeSH terms

  • Amino Acid Sequence
  • Blotting, Western
  • Chromatography, High Pressure Liquid / methods
  • Electrophoresis, Polyacrylamide Gel / methods
  • Endopeptidases / metabolism*
  • Glutathione Transferase / chemistry
  • Glutathione Transferase / genetics
  • Glutathione Transferase / metabolism
  • Kinetics
  • Mass Spectrometry / methods
  • Protein Structure, Secondary
  • Proteins / chemistry*
  • Proteins / genetics
  • Proteins / metabolism*
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism

Substances

  • Proteins
  • Recombinant Fusion Proteins
  • Glutathione Transferase
  • Endopeptidases