High-throughput methods for the large-scale analysis of gene function by transposon tagging

Methods Enzymol. 2000:328:550-74. doi: 10.1016/s0076-6879(00)28418-8.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • DNA Transposable Elements*
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Gene Library
  • Integrases / metabolism
  • Molecular Sequence Data
  • Mutagenesis, Insertional / methods*
  • Oligonucleotide Array Sequence Analysis
  • Plasmids
  • Recombinant Fusion Proteins / analysis
  • Recombinant Fusion Proteins / biosynthesis
  • Saccharomyces cerevisiae / genetics
  • Sequence Tagged Sites*
  • Viral Proteins*
  • beta-Galactosidase / analysis
  • beta-Galactosidase / genetics*

Substances

  • DNA Transposable Elements
  • Recombinant Fusion Proteins
  • Viral Proteins
  • Cre recombinase
  • Integrases
  • beta-Galactosidase