Development of shuttle vectors for spirochetes

J Mol Microbiol Biotechnol. 2000 Oct;2(4):443-5.


Constructions of Escherichia coli-spirochete shuttle vectors are based on naturally occurring plasmids, broad host range plasmids or bacteriophages. This review primarily focuses on genetic tools for Treponema denticola which is associated with periodontal diseases. The T. pallidum FlaA protein, E. coli beta-galactosidase, and the green fluorescent protein were successfully expressed in T. denticola from a shuttle vector system.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Flagellin / genetics
  • Genes, Reporter
  • Genetic Vectors*
  • Humans
  • Replication Origin
  • Spirochaetales / genetics*
  • Spirochaetales Infections / microbiology
  • Treponema / genetics


  • Flagellin
  • flaA protein, bacteria