Comparative study of biphasic calcium phosphate ceramics impregnated with rhBMP-2 as bone substitutes

J Biomed Mater Res. 2001 Jan;54(1):129-38. doi: 10.1002/1097-4636(200101)54:1<129::aid-jbm16>;2-d.


We investigated pellet-shaped implants prepared from biphasic calcium phosphate (BCP) ceramics with five different ratios of hydroxyapatite (HAP) to beta-tricalcium phosphate (beta-TCP) to evaluate these ceramics as bone substitutes. BCP ceramics impregnated with different doses of recombinant human bone morphogenetic protein 2 (rhBMP-2) (1, 5, and 10 microg) were used for experimental purposes and ceramics without rhBMP-2 were used for control. The pellets were implanted under the pericranium in adult Wistar male rats and were harvested 8 weeks after implantation. The retrieved pellets were then examined radiologically, histologically, and histomorphometrically. The results revealed that the pellets treated with rhBMP-2 exhibited new bone and bone marrow, whereas control pellets produced fibrous connective tissues. The formation of new bone induced by rhBMP-2 was dose dependent. The extent of bone and bone marrow formation and the degree of resorption of the ceramic particles were significantly higher in the pellets composed of 25% HAP-75% TCP. In this study, bioresorption of the ceramic produced favorable conditions for rhBMP-2-induced bone formation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Animals
  • Biocompatible Materials
  • Bone Morphogenetic Protein 1
  • Bone Morphogenetic Proteins / chemistry*
  • Bone Substitutes / chemistry*
  • Bone and Bones / cytology
  • Bone and Bones / diagnostic imaging
  • Calcium Phosphates / chemistry*
  • Ceramics / chemistry*
  • Durapatite / chemistry
  • Male
  • Materials Testing
  • Metalloendopeptidases / chemistry*
  • Radiography
  • Rats
  • Rats, Wistar
  • X-Ray Diffraction


  • Biocompatible Materials
  • Bone Morphogenetic Proteins
  • Bone Substitutes
  • Calcium Phosphates
  • beta-tricalcium phosphate
  • Durapatite
  • Metalloendopeptidases
  • BMP1 protein, human
  • Bmp1 protein, rat
  • Bone Morphogenetic Protein 1
  • tricalcium phosphate