Structure of mouse preproendothelin-3 and phylogenetic analysis of the endothelins

J Cardiovasc Pharmacol. 2000 Nov;36(5 Suppl 1):S1-4. doi: 10.1097/00005344-200036051-00003.


Endothelin (ET)-related peptide hormones, ET-1, vasoactive intestinal contractor (VIC), ET-2 and ET-3, have multiple physiological roles including vasoconstriction. To reveal the structural diversity of the precursor proteins of the ET family, cDNAs, cross-hybridizing with ET-1 and VIC probes, were cloned from the mouse intestine library. The deduced protein is a 214-amino acid precursor of mouse ET-3, preproendothelin-3 (PPET-3), which is the counterpart of the hypothalamus-, not placenta-, derived human PPET-3. Sequence identities of PPET-3 amino acids of mouse with human and rat are 65% over 194 amino acids and 65% over 214 amino acids. Phylogenetic analysis of the precursor proteins for ET-1, VIC and ET-3 suggest that members of the ET family are distantly related and probably descended from a common ancestral gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Endothelin-3 / genetics
  • Endothelins / chemistry*
  • Endothelins / genetics*
  • Humans
  • Intestinal Mucosa / metabolism
  • Mice
  • Molecular Sequence Data
  • Phylogeny
  • Protein Precursors / chemistry*
  • Rats


  • Endothelin-3
  • Endothelins
  • Protein Precursors
  • preproendothelin-3

Associated data

  • GENBANK/AB038561