Integration-proficient Pseudomonas Aeruginosa Vectors for Isolation of Single-Copy Chromosomal lacZ and Lux Gene Fusions

Biotechniques. 2000 Nov;29(5):948-50, 952. doi: 10.2144/00295bm04.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Artificial Gene Fusion / methods
  • Bacterial Proteins*
  • Cell Division
  • Chromosomes, Bacterial / genetics*
  • Cloning, Molecular / methods
  • Gene Dosage*
  • Genes, Reporter
  • Genetic Vectors / genetics*
  • Lac Operon / genetics*
  • Metalloendopeptidases / genetics
  • Promoter Regions, Genetic / genetics
  • Pseudomonas aeruginosa / genetics*
  • Recombination, Genetic / genetics*
  • Transcription, Genetic
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism

Substances

  • Bacterial Proteins
  • beta-Galactosidase
  • Metalloendopeptidases
  • pseudolysin, Pseudomonas aeruginosa