Blastocystis elongation factor-1alpha: genomic organization, taxonomy and phylogenetic relationships

Parasitology. 2000 Aug;121 ( Pt 2):135-44. doi: 10.1017/s0031182099006113.


The elongation factor-1 alpha (EF-1alpha) is a highly conserved ubiquitous protein that is involved in translation and is desirable for use in phylogenetic studies on Blastocystis, an enigmatic intestinal parasite with a contentious taxonomic position. In the present study, a PCR product (BEalpha) that codes for a major part of the coding region of the EF-lalpha protein was amplified. Genome walking experiments together with cloning were implemented to elucidate the 5' and 3' ends of the EF-1alpha gene of the human isolate, Blastocystis hominis C. The genomic organization and the potential transcription factor binding sites of the 5' end of B. hominis C EF-1alpha were identified. A comparative study on the deduced amino acid sequences of BEalpha of 13 Blastocystis isolates from various hosts was done to evaluate the phylogenetic relationship among the species. A phylogenetic reconstruction analysis with other eukaryotic EF-1alpha sequences was carried out to trace the phylogenetic position of Blastocystis among eukaryotic organisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blastocystis Infections
  • Blastocystis hominis / chemistry
  • Blastocystis hominis / classification
  • Blastocystis hominis / genetics*
  • Chromosome Walking
  • Cloning, Molecular
  • DNA Primers / chemistry
  • DNA, Protozoan / chemistry
  • DNA, Protozoan / isolation & purification
  • Molecular Sequence Data
  • Peptide Elongation Factor 1 / genetics*
  • Phylogeny*
  • Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid


  • DNA Primers
  • DNA, Protozoan
  • Peptide Elongation Factor 1