The open reading frame of the Na(+)-dependent glutamate transporter GLAST-1 is expressed in bone and a splice variant of this molecule is expressed in bone and brain

FEBS Lett. 2000 Nov 17;485(1):13-8. doi: 10.1016/s0014-5793(00)02175-x.

Abstract

Previously we have reported expression of an mRNA with homology to the Na(+)-dependent glutamate transporter, GLAST-1, in bone. Here we demonstrate that the complete open reading frame of GLAST-1 mRNA and corresponding 69 kDa protein are expressed in rat bone in vivo. We have also discovered a novel splice variant (GLAST-1a), lacking exon 3, expressed in rat bone and brain. A 55 kDa protein detected by anti-GLAST antibody in rat cerebellum corresponds to the molecular weight of unglycosylated GLAST-1a. This has led us to propose that GLAST-1a has an opposite orientation in the cell membrane to GLAST-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / genetics*
  • Alternative Splicing
  • Amino Acid Transport System X-AG
  • Animals
  • Base Sequence
  • Bone and Bones / chemistry*
  • Brain Chemistry*
  • Cerebellum / chemistry
  • Deoxyribonuclease EcoRI / metabolism
  • Exons
  • Gene Expression*
  • Glycosylation
  • Molecular Sequence Data
  • Open Reading Frames*
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • RNA, Messenger / chemistry
  • Rats
  • Rats, Wistar

Substances

  • ATP-Binding Cassette Transporters
  • Amino Acid Transport System X-AG
  • RNA, Messenger
  • Deoxyribonuclease EcoRI