Caenorhabditis elegans Chk2-like gene is essential for meiosis but dispensable for DNA repair

FEBS Lett. 2000 Nov 17;485(1):35-9. doi: 10.1016/s0014-5793(00)02178-5.

Abstract

A Chk2-like gene was identified in the genome of Caenorhabditis elegans. The putative gene product, termed Ce-chk-2 consists of 450 amino acid residues, and shows good homology with the Chk2/Cds1 gene family. The results of RNA-mediated interference (RNAi) indicated that the F1 generation from dsRNA injected animals grew to adulthood, but approximately 95% of their eggs (F2) died during early embryogenesis. Among the few surviving progeny, males (XO animals) arose at an abnormally high frequency (30%). In addition, 12 univalents were observed in full grown oocytes of the F1, while six bivalents were normally observed in wild-type oocytes. Ce-chk-2 gene expression increased in the adult stage, and their expression level decreased in the glp-4 mutant, which is defective in germ line proliferation. The radiation sensitivity of F1 embryos carrying Ce-chk-2 RNAi was not significantly affected.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Caenorhabditis elegans / embryology
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / radiation effects
  • Checkpoint Kinase 2
  • DNA Repair / genetics*
  • Gene Expression
  • Humans
  • Meiosis / genetics*
  • Molecular Sequence Data
  • Protein Kinases / chemistry
  • Protein Kinases / genetics*
  • Protein-Serine-Threonine Kinases*
  • RNA, Messenger / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Transfection

Substances

  • RNA, Messenger
  • Protein Kinases
  • Checkpoint Kinase 2
  • CHEK2 protein, human
  • Protein-Serine-Threonine Kinases

Associated data

  • GENBANK/AB049441
  • GENBANK/AL117207
  • GENBANK/AV177078
  • GENBANK/AV188394